Studies on 5-Fluorouracil-containing Ribonucleic Acid
نویسندگان
چکیده
منابع مشابه
Properties of 5-fluorouracil-containing ribonucleic acid and ribosomes from Bacillus subtilis.
Growth of a strain of Bacillus subtilis that requires uracil, thymine, adenine, and tryptophan in the presence of 5-fluorouracil (FU) results in the synthesis of ribonucleic acid (RNA) and ribosomes in which 55 to 65% of the RNA uracil has been replaced by the fluorine derivative. Examination of analogue-containing ribosomes by sucrose density gradient centrifugation and thermal denaturation st...
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The effects of cycloheximide and 5-fluorouracil on the formation of low-molecular-weight ribonucleic acid (RNA) in yeast were investigated. Both compounds were found to affect the synthesis of low-molecular-weight RNA of ribosomal origin more than transfer RNA but less than the high-molecular-weight ribosomal RNA. 5-Fluorouracil-containing transfer RNA was separated from normal transfer RNA by ...
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5-Fluorouracil, at lop4 M concentrations, inhibited ribosomal RNA maturation only slightly in Novikoff hepatoma cells growing in suspension culture. On a molar basis, 5-fluorouridine was a much more potent inhibitor of the maturation process. The difference between the effectiveness of 5-fluorouracil and 5-fluorouridine is probably a result of the greater transport and incorporation of the latt...
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A procedure for the isolation and purification of bacteriophage T34nduced RNA polymerase from T34nfected Escherichia coli cells is presented. The procedure described leads to the isolation of polymerase preparation that displays a single protein band by polyacrylamide gel electrophoresis and is free of detectable ribonuclease and deoxyribonuclease activities. Other enzyme activities absent from...
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A line of Gardner lymphosarcoma resistant to 5-fluorouracil (FU)' was derived by treating the drug-sensitive line with FU for @1 selection passages. There was a signifi cant decrease in orotic acid decarboxylating enzyme activity in the resistant tumor line as compared with that in the drug-sensitive line. A partially purified preparation of OMP-pyrophosphorylase from drug-sensitive tumor cells...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1969
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63464-7